Cell Culture and Hybridoma
Biology 6180
Spring Semester 2003
 
Dr. Tom McConnell (Office: BN-305; phone 328-4244)
Office Hours: Office hours: 10:00-11:00 AM  M-Th
Lecture: Room: BN106; Time: 1:00-1:50PM Mon
Laboratory  BIOL 6181; Time: 2:00-5:00PM MW,  BN-302

BIOL 6180 provides an introduction to the principles and mechanisms of producing monoclonal antibodies.

Grading Policy and Exam Procedures
Grading: 34% of the grade for the course will be based on the lecture midterm and final exams. The remaining 66% of the grade for the course will be based on the laboratory evaluation of each individual as determined by the laboratory instructor (details to be given in the laboratory section).

Prerequisites
Immunology BIOL 5190
 

Lecture Schedule
Lecture 1: Introduction
Lecture 2: Clonal selection theory and monoclonal antibody
Lecture 3: Antigen
Lecture 4: Cell fusion and cell selection mechanisms
Lecture 5: ELISA screening techniques, and cloning procedures
Lecture 6: Alternative immunoassays
Lecture 7: Antibody purification techniques
Lecture 8: Peptides and engineered antigen
Lecture 9: Genetically engineered antibodies
Lecture 10: Genetically engineered antibodies
Lecture 11-15: Discussion of topical basic science research papers


Calendar Schedule of Class Events
Exam 1: Midterm, February 26 (Wednesday!)
Final Exam: May 2 (Friday),  11:00 AM-1:00 PM

 Cell Culture and Hybridoma Links


Americans with Disabilities Act

East Carolina University seeks to fully comply with the Americans with Disabilities Act (ADA). Students requesting accommodations based on a covered disability must go to the Department for Disability Support Services, located in Brewster A-114, to verify the disability before any accommodations can occur. The telephone number is 252-328-6799.


 

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