Chapter 33

Protein Synthesis and Degradation

to accompany

Biochemistry, 2/e

by

Reginald Garrett and Charles Grisham

All rights reserved. Requests for permission to make copies of any part of the work should be mailed to: Permissions Department, Harcourt Brace & Company, 6277 Sea Harbor Drive, Orlando, Florida 32887-6777

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EfTu most abundant protein in E. coli

Requirements for elongation:

1) 70S complex with A site occupied by peptidyl-tRNA (or initiator tRNA, resembling peptidyl-tRNA)

2) Aminoacyl-tRNAs

3) EFs

4) GTP