Slide 58 of 62
Notes:
First step goes through an adenylate intermediate like tRNA aminoacylation does
Lys e-amino groups are most often targeted by the ligase (E3). Proteins that are selected 1) have free amino terminus 2) not Met, Ser, Ala, Thr, Val, Gly, Cys
Most susceptible proteins are secreted proteins, with end being exposed after transport into the ER
PEST sequence-proteins are also degraded readily (Pro, Glu, Ser, Thr)
