CURRICULUM VITAE

Cindy L. Putnam-Evans


CURRENT POSITION:

Associate Professor,
Biology Department
N-209 Howell Science Complex
East Carolina University
Greenville, NC 27858
Phone: 919-328-4811
Fax: 919-328-4178
E-mail: evansc@mail.ecu.edu

EDUCATION:
 

Year

Degree

Institution

Major Area

1978 

B.S. 

Gardner-Webb College 

Biology 

1982 

M.S. 

Clemson University 

Biochemistry 

1988 

Ph.D. 

University of Georgia 

Biochemistry 

 

TITLE OF MASTER'S THESIS:

"The Effects of 5-Hydroxytryptamine on a Clonal Line of B16 Melanoma" Dr. Carolyn S. Brown, major professor

 

TITLE OF DOCTORAL DISSERTATION:

"Purification and Characterization of a Calcium- Dependent Protein Kinase (CDPK) From Soybean" Dr. Milton J. Cormier, major professor

 

HONORS AND AWARDS:

1977 Alpha Chi Honor Society, Gardner-Webb College

1978 Paul J. Stacy Biology Award, Gardner-Webb College

1988 Graduate Student Research Presentation Award, Annual Meeting, Southeast Section of the American Society of Plant Physiologists, Blacksburg, VA

1994 Vice Chancellor's Summer Research/Creative Activity Stipend for Summer, East Carolina University

1995 College Research Award, College of Arts and Sciences, East Carolina University

1997 Helms Research Award, ECU Chapter of Sigma Xi

 

PROFESSIONAL SOCIETIES:

American Society of Plant Physiologists (1998-1999 Secretary/Treasurer-; 1999-2000 Vice Chair-Southern Section; 2000-2001 Chair-Southern Section; 2001-2003 Executive Committee-Southern Section)

North Carolina Plant Molecular Biology Consortium

North Carolina Academy of Sciences (1997-1998 Vice President)

Sigma Xi (ECU Chapter Sec./Treas. 2001-2003; President Elect 2003-2004; President 2004-2005)

 

POSITIONS HELD:

1978-79 Instructor, Gardner-Webb College, Boiling Springs, NC

1979 Instructor, Cleveland Community College, Shelby, NC

1979-82 Graduate Teaching Assistant, Clemson Univ., Clemson, SC

1982-84 Research Technician, University of Georgia, Athens, GA

1984-88 Graduate Research Assistant, Univ. of Georgia, Athens, GA

1988-89 Postdoctoral Research Associate, USDA-ARS, Richard Russell Research Center, Athens, GA

1989-93 Postdoctoral Research Associate, Botany Dept., Louisiana State University, Baton Rouge,LA

1993-1999 Assistant Professor, Biology Dept., East Carolina University, Greenville, NC

1999-now Associate Professor, Biology Dept., East Carolina University, Greenville, NC

 

PUBLICATIONS:

  1. R.C. Hart, R.E. Hice, H. Charbonneau, C. Putnam-Evans, and M.J. Cormier (1983). Preparation and Properties of Calcium-Dependent Resins with Increased Selectivity for Calmodulin. Anal.Biochem. 13: 208-220.
  2. M.J. Cormier, A. Harmon, and C. Putnam-Evans (1985). Ca2+- Dependent Regulation of NAD Kinase and Protein Phosphorylation in Plants. In "Calmodulin: Antagonists and Cell Function", H. Hidaka and D.J. Hartshorne, eds. Academic Press, Orlando, FL, pp. 445- 456.
  3. C.L. Putnam-Evans, A.C. Harmon, and M.J. Cormier (1986). Calcium-Dependent Protein Phosphorylation in Suspension-Cultured Soybean Cells. In "Molecular and Cellular Aspects of Calcium in Plant Development", A. Trewavas, ed., Plenum Press, NY, pp. 99-106.
  4. J. Preiss, M. Bloom, M. Morell, V.L. Knowles, W.C. Plaxton, T. Okota, R. Larsen, A.C. Harmon, and C. Putnam-Evans (1987). Regulation of Starch Synthesis: Enzymological and Genetic Studies. In "Tailoring Genes for Crop Improvement", G. Bruening, J. Harada, T. Kosuge, and A. Hollaender, eds., Plenum Press, NY, pp. 133-152.
  5. A.C. Harmon, C. Putnam-Evans, and M.J. Cormier (1987). A Calcium-Dependent But Calmodulin Independent Protein Kinase From Soybean. Plant Physiol. 83:830-837.
  6. C. Putnam-Evans, A.C. Harmon, B.A. Palevitz, M. Fechheimer, and M.J. Cormier (1989). Calcium-Dependent Protein Kinase is Localized with F-Actin in Plant Cells. Cell Motil. Cytoskel. 12:12-22.
  7. C. Putnam-Evans, A.C. Harmon, and M.J. Cormier (1990). Purification andCharacterization of a Novel Calcium-Dependent Protein Kinase From Soybean. Biochemistry. 29:2488-2495.
  8. J.F. Harper, M.R. Sussman, C. Putnam-Evans, H. Charbonneau, G.E. Schaller and A.C.Harmon (1991). A Novel Calcium-Dependent Protein Kinase With a Calmodulin-LikeRegulatory Domain, Science. 252:951-954.
  9. C. Putnam-Evans and T. Bricker (1992). Site-Directed Mutagenesis of the Cpa-1Protein of Photosystem II: Alteration of the Basic Residue Pair 384,385ARG to 384,385GLY Leads to a Defect Associated with the Oxygen Evolving Complex. Biochemistry. 31:11482-11488.
  10. C. Putnam-Evans and T. Bricker (1994). Site-Directed Mutagenesis of the CP-47 Protein of Photosystem II: Alteration of the Basic Residue 448ARG to 448GLY Prevents Assembly of Functional Photosystem II Centers under Chloride-Limiting Conditions. Biochemistry. 33:10770-10776.
  11. Putnam-Evans, C., Burnap, R., Wu, J., Whitmarsh, J. and Bricker, T.M. (1995) Site-Directed Mutagenesis of the CP 47 Protein of Photosystem II: Alteration of Conserved Charged Residues in the Domain 364E-440D. Biochemistry. 35:4046-4053.
  12. Putnam-Evans, C., Baker, John and Faith Burnett (1996) Characterization of a Yellow Mutant of Synechocystis. SAAS Bull. Biochem. Biotechnol. 9:17-24.
  13. Wu, Jituo, Putnam-Evans, C. and T. Bricker (1996) Site-Directed Mutagenesis of the CP47 Protein of Photosystem II: 167W in the Lumenally Exposed Loop A is required for Photosystem II Assembly and Stability. Plant Molecular Biology. 32:537-542.
  14. Putnam-Evans, C., Wu, Jituo and Bricker, T.M. (1996) Site-Directed Mutagenesis of the CP43 Protein of Photosystem II:Alteration of Conserved Charged Residues Which Lie Within Lethal Deletions of the Large Extrinsic Loop E. Plant Molecular Biology. 32:1191-1195.
  15. Putnam-Evans, C. and Bricker, T.M. (1997) Site-Directed Mutagenesis of the Basic Residue 321K to 321G in the CP47 Protein of Photosystem II Alters the Chloride Requirement For Growth and Oxygen-Evolving Activity in Synechocystis 6803. Plant Molecular Biology. 34:455-463.
  16. Goldfarb, Nathan, Knoepfle, Nicholas and Putnam-Evans, Cindy (1997). Construction of a psbC Deletion Strain in Synechocystis 6803. SAAS Bull. Biochem. Biotechnol. 10:1-6.
  17. McKnight, S.L., Oliver, Melvin J. and Putnam-Evans, C. (1997). Nucleotide Sequence of a Full-Length cDNA (Accession Number U82087) Encoding a Calmodulin-Like Domain Protein Kinase (CDPK) From the Moss Tortula ruralis. Plant Physiology. 115:313.
  18. Qian, M., Al-Khaldi, S., Putnam-Evans, C., Bricker, T. and R.L. Burnap (1998). Photoassembly of the PSII (Mn)4 Cluster in Site-Directed Mutants Impaired in the Binding of the Manganese-Stabilizing Protein. Biochemistry. 36:15244-15252.
  19. Bricker, Terry M., Putnam-Evans, Cindy and Wu, Jituo. (1998). Directed Mutagenesis in Photosystem II:Analysis of the CP 47 Protein. Methods in Enzymology. 297:320-337.
  20. Knoepfle, Nicholas, Bricker, Terry M. and Cindy Putnam-Evans (1999). Site-Directed Mutagenesis of Basic Residues 305R and 342R in the CP 43 Protein of Photosystem II Affects Oxygen-Evolving Activity in Synechocystis 6803. Biochemistry.  38:1582-1588.
  21. Rosenberg, Christina, Christian, Julie, Bricker, Terry M. and Cindy Putnam-Evans (1999). Site-Directed Mutagenesis of Glutamate Residues in the Large Extrinsic Loop of the Photosystem II Protein CP 43 Affects Oxygen-Evolving Activity and PSII Assembly. Biochemistry, 38:15994-16000.
  22. Anderson, L.B., Maderia, M., Ouelette, A.J.A., Putnam-Evans, C., Higgins, L., Krick, T., MacCoss, M.J., Lim, H., Yeats, J.R. and Barry, B.A. (2002).  Post-Translational Modifications in the CP43 Subunit of the Photosynthetic Water-Oxidizing Complex.  Proc. Natl. Acad. Sci.  99:14676-14681.
  23. Young, A., McChargue, M., Frankel, L.K., Bricker, T.M., and Putnam-Evans, C.  (2002)  Alterations of the Oxygen-Evolving Apparatus Induced by a 305Arg to 305Ser Mutation in the CP43 Protein of Photosystem II from Synechocystis sp. PCC6803 under Chloride-Limiting Conditions.  Biochemistry. 41:15747-15753.
  24. Bricker, T.M., Young, A., Frankel, L.K., and Putnam-Evans, C.  (2002)  Introduction of the 305Arg to 305Ser Mutation in the Large Extrinsic Loop E of the CP43 Protein of Synechocystis sp. PCC 6803 Leads to Loss of Cytochrome C550 Binding to Photosystem II.  Biochimica Biophysica Acta.  1556:92-96.
  25. Eaton-Rye, J.J. and Putnam-Evans, C.  (2005)  “The Proximal Antenna Proteins CP47 and CP43” in Photosystem II:The Water Plastoquinone Oxido-Reductase of Photosynthesis.  Chapter 22: Advances in Photosynthesis and Respiration Series, Kluwer Academic Publishers, in press.
  26. Booth, D., Sirochman, R., Richardson, D.C., Richardson, J.S., Weiner, S.W., Farwell, M., Putnam-Evans, C., and Bateman, R.  (2005)  Assessment of Molecular Construction in Undergraduate Biochemistry.  Journal of Chemical Education, in press.
  27. Ananyev, G., Nguyen, T., Sequeira, S, Dasgupta, J., Putnam-Evans, C. and Dismukes, C.  (2005)  Carbonate Functions in the Photosynthetic O2-Evolving Complex:  Mutagenesis of CP43-Arginine-357 Abolishes a Specific Binding Site for Bicarbonate.  (2005) Photochemical & Photobiological Sciences, submitted.
  28. Lim, K.H., Le, Y.T.H., Collver, H., Putnam-Evans, C., and Kenney, J.  (2005) A Novel a-Helical Amyloidogenic Intermediate State, in preparation.
  29. Gaines, R., Arno, J., Carroll, R., Bricker, T.M. and Putnam-Evans, C.  (2005)  The D306N Mutation in the CP43 Protein of Photosystem II Leads to an Altered Chloride Requirement for Growth and Oxygen Evolving Activity, in preparation.

 

ABSTRACTS:

  1. C. Putnam and C.S. Brown. (1980). The Effect of Serotonin on Growth and Differentiation of Murine B16C3 Melanoma Cells. American Society for Microbiology, Miami, Abstracts.
  2. C.L. Putnam and C.S. Brown (1980). Demonstration of Monoamine Oxidase Activity in B16C3 Murine Melanoma Cells. Fed. Proc., 39:2944.
  3. C.S. Brown and C. Putnam (1982). The Effects of Serotonin of Morphology, DNA Synthesis and RNA Synthesis in Murine Melanoma Cells. SC Academy of Sciences, Clemson University.
  4. C.L. Putnam-Evans and M.J. Cormier (1984). Ca2+-Dependent Protein Kinase Activity in a Plant Cell Line. Fed. Proc., 43:296.
  5. M.J. Cormier, A.C. Harmon, and C.L. Putnam-Evans (1984). Ca2+-Dependent Phosphorylation of NAD and Protein in Plants. Third International Congress on Cell Biology, Proceedings.
  6. C. Putnam-Evans and M.J. Cormier (1985). Identification of a Ca2+-Dependent Protein Kinase From Soybean Suspension Cultures. First International Congress of Plant Molecular Biology, Savannah, GA, Abstracts.
  7. C. Putnam-Evans, A.C. Harmon and M.J. Cormier (1987). A New Class of Protein Kinase: The Ca2+- Dependent, Calmodulin- and Phospholipid-Independent Protein Kinase From Soybean. Plant Physiology Supplement, 83:51.
  8. S.B. Wilde, F.A. Lashley, C. Putnam-Evans, and C.S. Brown (1988). Syntheses and Degradation of Biogenic Amines in B16 Murine Melanoma Cells and Normal Melanocytes. American Chemical Society Meeting, Toronto, Canada.
  9. A.C. Harmon, C. Putnam-Evans, B.A. Palevitz, M. Fechheimer, and M.J. Cormier (1988). Calcium-Dependent Protein Kinase is Associated With Actin Fibers in Plant Cells. First International Workshop on Second Messengers and Phosphoinositides in Plants. West Lafayette, IN, Abstracts.
  10. A.C. Harmon, C. Putnam-Evans, B.A. Palevitz, M. Fechheimer, and M.J. Cormier (1988). Calcium-Dependent Protein Kinase is Associated With Actin Fibers in Plant Cells. Plant Physiology Supplement 84(4):64.
  11. C. Putnam-Evans, A. Harmon, B. Palevitz, and M.J. Cormier (1988). Immunocytochemical Localization ofthe Calcium-Dependent Protein Kinase From Soybean. Southeast Regional Meeting of the American Societyof Plant Physiologists, Blacksburg, VA, Abstracts.
  12. A.C. Harmon, M. Fechheimer, and C. Putnam-Evans (1988). Immunocytochemical Localization of Calcium-Dependent Protein Kinase in HeLa Cells. J. Cell. Biol. 107:285a.
  13. C. Putnam-Evans and T. Bricker (1990). In Vitro Mutagenesis in the Large Extrinsic Loop Region of CPa-1: A Progress Report. 1990 Gordon Conference-Biochemical Aspects of Photosynthesis.
  14. C. Putnam-Evans and T. Bricker (1991). Site-Directed Mutagenesis of the psbB Gene of PSII: Alteration of the Highly Conserved Basic Residue Pairs in the Large Extrinsic Loop. Plant Phys. Suppl. 96(1):115.
  15. C. Putnam-Evans and T. Bricker (1991). Site-Directed Mutagenesis in the Large Extrinsic Loop of CPa-1: Alteration of Conserved Basic Residue Pairs Modifies Photosystem II Activity. Midwest Photosynthesis Conference, Abstracts.
  16. C. Putnam-Evans and T. Bricker (1994). Site-Directed Mutagenesis of the CP 47 Protein of Photosystem II: Cumulative Effects of Mutations at Positions 384 and 385 in the Large Extrinsic Loop. American Society of Plant Physiologists (Southern Section) Conference, Abstracts.
  17. C. Putnam-Evans, Ulla Godwin, Estelle Hrabak, John Satterlee and Mike Sussman. (1995). Monoclonal Antibodies to Specific Isoforms of Calmodulin-Like Domain Protein Kinase. NC Plant Molecular Biology Consortium Annual Meeting, Abstracts.
  18. C. Putnam-Evans and T. Bricker (1996). Site-Directed Mutagenesis of the CP-47 Protein of Photosystem II: Mutations Affecting the Chloride Requirement of Photosystem II. American Society of Plant Physiologists (Southern Section) Conference Abstracts.
  19. C. Putnam-Evans and T. Bricker (1996). Site-Directed Mutagenesis of the Basic Residue K321 to G321 in the CP 47 Protein of Photosystem II Alters the Chloride Requirement for Growth and Oxygen-Evolving Activity in Synechocystis 6803. Gordon Conference-Biochemical Aspects of Photosynthesis.
  20. Michelle Kosovak and Cindy Putnam-Evans (1996). Site-Directed Mutagenesis of N-Terminal Threonine Residues in the Photosystem II Protein CP 43. NC Plant Molecular Biology Consortium Annual Meeting, Abstracts.
  21. Susan McKnight and Cindy Putnam-Evans (1996). Cloning of Calmodulin-Like Domain Protein Kinase (CDPK) From the Moss Tortula ruralis. NC Plant Molecular Biology Consortium Annual Meeting, Abstracts.
  22. Nathan Goldfarb and C. Putnam-Evans (1997). Construction and Analysis of the CP 43 Protein Large Extrinsic Loop Deletion Strain in Synechocystis 6803. Third Annual Graduate Student Research Symposium, East Carolina University, April 15, 1997, Abstracts.
  23. Nathan Goldfarb, Nicholas Knoepfle and C. Putnam-Evans (1998). Random Mutagenesis of 185T to 185I in the Hydrophilic Loop C of the CP 43 Protein of Photosystem II. Annual Meeting American Society of Plant Physiologists-Southern Section, Roanoke, VA, Abstracts.
  24. Susan McKnight, Mel Oliver and C. Putnam-Evans (1998). Isolation and Characterization of a Calmodulin-Like Domain Protein Kinase cDNA from the Moss Tortula ruralis. Annual Meeting American Society of Plant Physiologists-Southern Section, Roanoke, VA, Abstracts.
  25. Nicholas Knoepfle and C. Putnam-Evans (1998). Site-Directed Mutagenesis of the CP 43 Protein of Photosystem II:Alteration of Conserved Charged Residues 305R and 342R Affects PSII Activity. Annual Meeting American Society of Plant Physiologists-Southern Section, Roanoke, VA, Abstracts.
  26. Christina Rosenberg, Nathan E. Goldfarb, Nicholas Knoepfle and Cindy Putnam-Evans (1998).  Effects of Site-Directed Mutagenesis on Conserved, Charged Amino Acid Residues in the Large Extrinsic Loop of the CP43 Protein of Photosystem II.  Annual Meeting American Society of Plant Physiologists-Southern Section, Roanoke, VA, Abstracts.
  27. Nathan Goldfarb, Nicholas Knoepfle and C. Putnam-Evans (1998). Random Mutagenesis of 185T to 185I in the Hydrophilic Loop C of the CP 43 Protein of Photosystem II. Annual Meeting of the North Carolina Academy of Sciences, Greensboro, NC, Abstracts.
  28. Nathan Goldfarb, Nicholas Knoepfle and C. Putnam-Evans (1998). The CP 43 Protein of Photosystem II in Synechocystis 6803: Random Mutagenesis of the Large Extrinsic Loop. FASEB Journal. 12(8):A1443.
  29. Nicholas Knoepfle, Terry M. Bricker and C. Putnam-Evans (1998). Site-Directed Mutagenesis of the CP 43 Protein of Photosystem II:Alteration of Conserved Charged Residues 305R and 342R Affects PSII Activity. 22nd Annual Midwest Photosynthesis Conference, Marshall, IN, October 27-29, Abstracts.
  30. Christina Rosenberg, Julie Christian, Terry Bricker and Cindy Putnam-Evans (1999). Site-Directed Mutagenesis of Glutamate Residues in the Large Extrinsic Loop of the Photosystem II Protein CP 43 Affects Oxygen-Evolving Activity and PSII Assembly. Annual Meeting American Society of Plant Physiologists-Southern Section, Baton Rouge, LA, March 27-29.
  31. Christina Rosenberg, Julie Christian, Terry Bricker and Cindy Putnam-Evans (1999). Site Directed Mutagenesis of Glutamate Residues in the large Extrinsic Loop of the CP 43 Protein of Photosystem II Affects Oxygen-Evolving Activity and PSII Assembly. Gordon Conference-Biochemical Aspects of Photosynthesis, Henniker, NH, July 13-18.
  32. Andrew Young and Cindy Putnam-Evans.  Mutagenesis of the Residue 305R to 305S in the CP43 Protein of Photosystem II Alters the Chloride Requirement for Growth and Oxygen Evolving Activity.  American Society of Plant Physiologists-Southern Section Annual Meeting, Chattanooga, TN, March 25-27, 2000.
  33. Christy Glossip, Chris Rosenberg, Julie Christian, Nick Knoepfle and Cindy Putnam-Evans.  Site-Directed Mutagenesis of Conserved, Charged Amino Acids Occurring Within Lethal Deletions of the Large Extrinsic Loop E of the Photosystem II Protein CP43.  American Society of Plant Physiologists-Southern Section Annual Meeting, Chattanooga, TN, March 25-27, 2000.
  34. Andrew Young and Cindy Putnam-Evans.  Mutagenesis of the Residue 305R to 305S in the CP43 Protein of Photosystem II Alters the Chloride Requirement for Growth and Oxygen Evolving Activity.  East Carolina University, Graduate Student Research Day, April 3, 2000.
  35. Andrew Young, Robin Gaines and Cindy Putnam-Evans.  Site-Directed Mutations in the CP43 Protein of Photosystem II Alter the Chloride Requirement for Growth and Oxygen Evolving Activity in Synechocystis 6803.  American Society of Plant Physiologists-Southern Section Annual Meeting, Raleigh, NC, March 24-26, 2001.
  36. Andrew Young, Brandon Burch and Cindy Putnam-Evans.  Directed Mutagenesis at Position 305 in the CP43 Protein of Photosystem II.  American Society of Plant Physiologists-Southern Section Annual Meeting, Athens, GA, March 2-4, 2002.
  37. Jaysen Arno, Sandy Machon and Cindy Putnam-Evans.  Site-Directed Mutagenesis of 285E to 285Q in the CP43 Protein of Photosystem II Affects the Chloride Requirement for Growth and Oxygen Evolution.  American Society of Plant Physiologists-Southern Section Annual Meeting, Athens, GA, March 2-4, 2001.
  38. Andrew Young, Miriam McChargue, Terry M. Bricker and Cindy Putnam-Evans.  Effects of Chloride Depletion on Oxygen-Evolving Activity in the R305S Mutant of the CP43 Protein of Photosystem II.  Photosynthesis Gordon Conference, June 16-21, 2002.
  39. Terry M. Bricker, Andrew Young, Laurie K. Frankel and Cindy Putnam-Evans.  Introduction of the 305Arg to 305Ser Mutation in the Large Extrinsic Loop E of the CP43 Protein of Synechocystis sp. PCC 6803 Leads to Loss of Cytochrome C550 Binding to Photosystem II.  Mid-West Photosynthesis Conference, Marshall, IN, October 27-29, 2002.
  40. Anderson, L.B., Maderia, M., Ouelette, A.J.A., Putnam-Evans, C., Higgins, L., Krick, T., MacCoss, M.J., Lim, H., Yeats, J.R. and Barry, B.A. (2002).  Post-Translational Modifications in the CP43 Subunit of the Photosynthetic Water-Oxidizing Complex.  Mid-West Photosynthesis Conference, Marshall, IN, October 27-29, 2002.
  41. Gaskins, Tara and Putnam-Evans, Cindy.  Mutations at Position 305 in the CP43 Protein of Photosystem II Affect the Calcium Requirement for Growth and Oxygen Evolving Activity in Synechocystis 6803.  Undergraduate Honor’s Research Day, East Carolina University, March 26, 2004.
  42. G. Ananyev, T. Nguyen, R. van Willigen, J. Dasgupta, C. Putnam-Evans and C. Dismukes.  Involvement of Arginine 357-CP43 in Donor Side Bicarbonate Effect of Photosystem II.  21st Annual Eastern Regional Photosynthesis Conference, Woods Hole, MA, April 16-18, 2004
  43. Cindy Putnam-Evans.  The Role of the CP43 Protein in Oxygen Evolution from Photosystem II.  American Society of Plant Physiologists-Southern Section Annual Meeting, Greenville, NC, March 12-14, 2005.

 

INVITED RESEARCH TALKS:

  1. Identification of a Ca2+-Dependent Protein Kinase in Soybean Suspension Cultures. First International Congress of Plant Molecular Biology, 1985, Savannah, GA.
  2. Immunocytochemical Localization of the Calcium-Dependent Protein Kinase From Soybean. 1988 Annual Meeting of the Southeast Section of the American Society of Plant Physiologists, Blacksburg, VA.
  3. Purification and Characterization of a Ca2+-Dependent Protein Kinase (CDPK) From Soybean. Oct. 4, 1988, Biochemistry Dept., North Carolina State University, Raleigh, NC.
  4. Biochemical Characterization of a Novel Calcium-Binding Protein Kinase. February 1990, Botany Department, Louisiana State University, Baton Rouge, LA.
  5. Site-Directed Mutagenesis of the CPa-1 Protein of Photosystem II. Dec. 4, 1992, Dept. of Biological Sciences, East Tennessee State University, Johnson City, TN.
  6. Site-Directed Mutagenesis of the CPa-1 Protein of Photosystem II. April 28, 1993, Biology Dept., East Carolina University, Greenville, NC.
  7. Site-Directed Mutagenesis of the CP-47 Protein of Photosystem II. Oct. 24, l994, Biochemistry Dept., East Carolina University School of Medicine, Greenville, N.C.
  8. Site-Directed Mutagenesis of the CP-47 Protein of Photosystem II. April 11, l994, Microbiology and Immunology Dept., East Carolina University School of Medicine, Greenville, N.C.
  9. Site-Directed Mutagenesis of the CP-47 Apoprotein of Photosystem II. September 23, l995, NC Plant Molecular Biology Consortium Annual Meeting, Moorehead City, NC.
  10. Site-Directed Mutagenesis of the CP-47 Protein of Photosystem II: Mutations Affecting the Chloride Requirement of Photosystem II. Feb. 5, l996, Southeastern Association of Agricultural Biologists Annual Meeting, Greensboro, N.C.
  11. Site-Directed Mutagenesis of the CP-47 Protein of Photosystem II: Mutations Affecting the Chloride Requirement of Photosystem II. Feb. 23, l996, Biology Dept., Western Carolina University, Cullowhee, N.C.
  12. Site-Directed Mutagenesis of the CP-47 Protein of Photosystem II: Mutations Affecting the Chloride Requirement of Photosystem II. March 6, l996, Botany Dept., North Carolina State University, Raleigh, N.C.
  13. Site-Directed Mutagenesis of the CP 47 Protein of Photosystem II. October 18, 1996, Botany Dept, Duke University, Durham, N.C.
  14. Site-Directed Mutagenesis of the CP 47 and CP 43 Proteins of Photosystem II. December 4, 1997, Dept. of Anatomy and Cell Biology, East Carolina University School of Medicine, Greenville, NC.
  15. Site-Directed Mutagenesis of the CP 43 Protein of Photosystem II:Alteration of Conserved Positively Charged Residues 305R and 342R Affects PSII Activity. October 23, 1998, Dept. of Physics, East Carolina University School of Medicine, Greenville, NC.
  16. Site-Directed Mutagenesis of the CP 43 Protein of Photosystem II:Alteration of Both Basic and Acidic Residues in the Large Extrinsic Loop Affects PSII Activity.  September 8, 1999, Biology Dept., East Carolina University.
  17. Site-Directed Mutagenesis of the CP 43 Protein of Photosystem II:Alteration of Both Basic and Acidic Residues in the Large Extrinsic Loop Affects PSII Activity.  September 10, 1999, Department of Cell Biology and Anatomy, East Carolina University School of Medicine.
  18. Site-Directed Mutagenesis in the CP43 Protein of Photosystem II:  Novartis/UNC-Greensboro sponsored symposium on “The Biochemistry of Light”, April 14, 2000.
  19. Effects of Chloride Depletion on Oxygen-Evolving Activity in Site-Directed Mutants of the CP43 Protein of Photosystem II.  Moreland Family Mini-Symposium:  Structure and Function of Photosystem II, Louisiana State University, June 14, 2002.
  20. Role of the CP43 Protein in Photosynthetic Oxygen Evolution, September 23, 2005, Dept. of Chemistry, UNC-Greensboro.

 

GRANT SUPPORT:

 

1.  Funded as P.I.: NSF Research Planning Grant "The Role of N-Terminal Phosphorylation of the Photosystem II Proteins CPa-2 and D2 as Probed by Site-Directed Mutagenesis".  National Science Foundation. $18,000, July 1, 1994-December 31, 1996

2.  Funded as Co-PI: Faculty Research Development Award from the College of Arts and Sciences (East Carolina University), $1,900, Spring 1995.

3.  Funded as Co-PI: "A Modern Protein Purification Course".  North Carolina Biotechnology Center. $88,927, February 1, 1996-January 1, l997

4.  Funded as PI: "Mutagenesis Studies to Probe the Function of the Large Extrinsic Loop of the CP43 Apoprotein of Photosystem II".   National Science   

Foundation. $180,000, March 1, 1996-March 1, l999.

5.  Funded as Co-PI: "Establishment of a Center for Science Education Computer Excellence".  East Carolina University Student Computer and Technology Fee Program. $98,000, May 14, 1996.

6.  Funded as P.I.:  “Interaction of Domains on CP43 with Components Required for Oxygen Evolution”.  National Science Foundation.  $200,432, April 1, 2000-March 31, 2004.

7.  Funded as Co-P.I.:  “Acquisition of Automated DNA Sequencing Equipment for East Carolina University”.  National Science Foundation.  $132,930, Sept. 1, 2000-Sept. 1, 2001.

8.  Funded as P.I.:  “Interaction of the Photosystem II Protein CP32 with Cytochrome c550”.  East Carolina University Research/Creative Activity Grant.  $10,000, May-August 2003.

9.  Funded as P.I.:  “Role of CP43 in Protein/Protein and Protein/Cofactor Interactions in Photosystem II”.  National Science Foundation.  $279,193, August 1, 2005-July 31, 2008.

 

Contributing Investigator:  “First Circular Dichroism Spectrometer for East Carolina University”.  North Carolina Biotechnology Center.  $57,531, 2002.

Contributing Investigator:  “Acquisition of Mass Spectrometers for East Carolina University”.  $503,248, Sept. 1, 2005.

 

PROFESSIONAL EXPERIENCE:

 

1994-present: Ad hoc reviewer for the following scientific journals: Physiologia Plantarum , Plant Physiology, and Biochemistry

Member of Graduate Student Awards Committee, North Carolina Chapter of the American Society for Microbiology annual meeting, Charlotte, NC, Nov. 1993

Chairman, Graduate Student Awards Committee, American Society for Plant Physiology (Southern Section) annual meeting, Knoxville, TN, March 18-20, 1995.

1995-1997: Member of Site-Selection Committee for the 1998 American Society for Plant Physiology (Southern Section) annual meeting.

1996-: Member of Editorial Board for the Journal of the Elisha Mitchell Society

Member of Graduate Student Awards Committee, American Society of Plant Physiologists-Southern Section annual meeting, Orlando, Fl, March 30-April 1, l996

1996-present: Ad Hoc reviewer for USDA and NSF competitive grants programs

2001:  Member of NSF Committee of Visitors for evaluation of Plant Genomics Program

2003:  Co-Organizer, Mid-West Photosynthesis Conference

2005:  Co-Organizer, American Society of Plant Biologists-Southern Section Annual Meeting

 

STUDENTS GRADUATED:

 

Michelle Kosovac, "Synechocystis 6803 Photosystem II Proteins CP43 and D2: The Role of the N-Terminal Threonine Residue as Probed by Oligonucleotide Site-Directed Mutagenesis". M.S. completed Sept. 1997.

Susan McKnight, "Isolation and Characterization of a Calmodulin-Like Domain Protein Kinase cDNA from the Moss Tortula ruralis." M.S. completed June 1998.

Nicholas Knoepfle, " Site-Directed Mutagenesis of the CP 43 Protein of Photosystem II:Alteration of Conserved Charged Residues 305R and 342R Affects PSII Activity." M.S. completed June 1998.

Nathan Goldfarb, " Random Mutagenesis of 185T to 185I in the Hydrophilic Loop C of the CP 43 Protein of Photosystem II." M.S. completed July, 1998.

Christina Rosenberg, "The CP 43 Protein of Photosystem II in Synechocystis 6803: Effects of Site-Directed Mutagenesis of Conserved Acidic Residues in the Large Extrinsic Loop." M.S. completed May 1999.

Christina Glossip, “Site-Directed Mutagenesis of Loop C and the Large Extrinsic Loop E of the Photosystem II Protein CP43 in the Cyanobacteruim Synechocystis 6803”, M.S. completed June 2000.

Andrew Young, “Characterization of the Effects of Chloride Depletion on Oxygen-Evolving Activity in the R305S Mutant of the CP43 Protein of Photosystem II”, M.S. completed December 2000.

Brandon Burch, “Characterization of the Effects of Chloride Depletion on Oxygen-Evolving Activity in the R305D and R305K Mutants of the CP43 Protein of Photosystem II”, M.S. completed November 2004.

CURRENT RESEARCH:

 

My lab is currently involved in a mutagenesis project designed to probe the structure/function of the chlorophyll-binding protein CP43.  CP43 is a component of the Photosystem II (PSII) complex of higher plants, green algae and cyanobacteria (blue-green algae). Photosystem II catalyzes the initial events of photosynthesis, i.e., the light-driven oxidation of water and reduction of plastoquinone.  Molecular oxygen is evolved as a by-product of these reactions.  CP43 plays a role in the stable assembly of PS II and in the oxygen-evolving process.  Using mutagenesis techniques, we have identified domains on the lumenal surface of CP43 that appear to interact, either directly or indirectly, with components of the oxygen-evolving complex, in particular, cytochrome c550 and the inorganic cofactors Cl- and Mn2+ that are required for oxygen evolution.  We are expanding the characterization of these mutants and are constructing and characterizing additional mutations at these sites in order to more clearly define the roles of these domains in PSII function.  We employ a combination of physiological, biochemical and biophysical techniques in the analysis of mutants.

 

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